Development of a Diagnostic Method Applicable to Various Serotypes of Hantavirus Infection in Rodents

Journal of Veterinary Medical Science

Jiro ARIKAWA³  Kumiko YOSHIMATSU³  Evgeniy A. TKACHENKO⁴  Keisuke YOSHIKAWA¹  Kentaro YOSHII¹  Takahiro SETO¹  Ngonda SAASA¹  Vyacheslav G. MOROZOV⁵  Leonid I. IVANOV²  Hiroaki KARIWA¹  Ikuo TAKASHIMA¹  Takahiro SANADA¹ 

[1] Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Kita–18 Nishi–9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan;Plague Control Station of Khabarovsk, Sanitarny Lane, Khabarovsk 680031, Russia;Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita–15 Nishi–7, Kita-ku, Sapporo, Hokkaido 060–0838, Japan;Chumakov Institute of Polyomyelitis and Viral Encephalitidis, Moscow 142782, Russia;Medical Company “Hepatolog” Incorporated, 3 Dachnaya Proseka St., 157, 443011 Samara, Russia

关键词: diagnosis;    ELISA;    hantavirus;    rodents;   

DOI  :  10.1292/jvms.12-0006

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Antigenic diversity among different hantaviruses requires a variety of reagents for diagnosis of hantavirus infection. To develop a diagnostic method applicable to various hantavirus infections with a single set of reagents, we developed an enzyme-linked immunosorbent assay (ELISA) using recombinant nucleocapsid proteins of three hantaviruses, Amur, Hokkaido, and Sin Nombre viruses. This novel cocktail antigen-based ELISA enabled detection of antibodies against Hantaan, Seoul, Amur, Puumala, and Sin Nombre viruses in immunized laboratory animals. In wild rodent species, including Apodemus, Rattus, and Myodes, our ELISA detected antibodies against hantaviruses with high sensitivity and specificity. These data suggest that our novel diagnostic ELISA is a useful tool for screening hantavirus infections and could be effectively utilized for serological surveillance and quarantine purposes.

【 授权许可】

Unknown