Bacteriostatic Activity of Whey Acidic Protein (WAP)

Journal of Veterinary Medical Science

Tokuko IWAMORI²  Naoko NUKUMI²  Hideaki TOJO²  Masamichi KUROHMARU³  Keitaro YAMANOUCHI⁴  Kikuji ITOH¹  Kunihiko NAITO²  Kiyoshi KANO² 

[1] Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Applied Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Laboratory of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: antimicrobial peptides;    antimicrobial resistance;    antimicrobial substance;   

DOI  :  10.1292/jvms.08-0331

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(7)We have previously reported the action of whey acidic protein (WAP) inhibiting the proliferation of mouse mammary epithelial cells in the experiments utilizing in vivo and in vitro systems. We report herein the bacteriostatic activity of WAP. Western blot analysis demonstrated successful isolation of WAP from whey fractions of rat milk by column chromatography. The WAP fraction inhibited the growth of Staphylococcus aureus JCM2413 in a dose-dependent manner, but did not inhibit the growth of Escherichia coli. The bacteriostatic activity of WAP was highest at pH 6.6 and was not affected by the presence of 150 mM NaCl. A scanning electron micrograph of bacteria treated with WAP exhibited the disruption of the bacterial cell walls.

【 授权许可】

Unknown