Glucose Uptake Activity in Murine Red Blood Cells Infected with Babesia microti and Babesia rodhaini

Journal of Veterinary Medical Science

Naoaki MATSUKI¹  Keishi ADACHI¹  Yoshimitsu FUKUDA¹  Takashi OHMORI¹  Kenichiro ONO¹  Satoshi TAMAHARA¹ 

[1] Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: Babesia microti;    Babesia rodhaini;    glucose uptake;    murine;    RBC;   

DOI  :  10.1292/jvms.66.945

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(1)The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC.

【 授权许可】

Unknown