A Short Term in Vitro Cultivation of Babesia rodhaini and Babesia microti

Journal of Veterinary Medical Science

Terumasa SHIMADA¹  Kenichi NAKADA¹  Sojin SHIKANO¹  Kenichiro ONO¹  Rie HASHIGUCHI¹ 

关键词: Babesia microti;    Babesia rodhaini;    cultivation;   

DOI  :  10.1292/jvms.57.955

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(1)In vitro cultivation of Babesia rodhaini (BR) and Babesia microti (BM) was attempted. When RPMI1640 was supplemented with 30 or 40% of non-treated fetal bovine serum (FBS), the gas mixture of 30% CO2-80% O2 best supported the growth of both parasites. Under this optimized condition, the percent parasitized erythrocytes peaked to approximately 4- and 2-times initial values for BR and BM, respectively. The cultivated parasites retained the infectivity to the host mice. BM showed the characteristic feature of division during cultivation. However, the lots of FBS will have to be taken into consideration, since the FBS lots were shown to give large varieties to the results. Selection of the appropriate FBS lot may yield the better growth of these protozoa.

【 授权许可】

Unknown