Antibiotic Furazolidone Induces CYP1A But Not CYP2E1 Subfamily in Rat Liver

Journal of Veterinary Medical Science

Yoshinori IKENAKA²  Noriaki SAKAI¹  Min HEEWON¹  Shoichi FUJITA¹  Tomomi KAMIYA¹  Mayumi ISHIZUKA¹  Nobuo SASAKI¹  Kentaro Q SAKAMOTO¹ 

[1] Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Division of Science for Inland Water Environment, Institute of Mountain Science, Shinshu University

关键词: CYP1A1;    cytochrome P450;    furazolidone;    mutagenicity;    rat;   

DOI  :  10.1292/jvms.70.223

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Furazolidone (FZ), one of the nitrofuran fungicides, is used as a veterinary medicine in the Middle and Far Eastern countries. In this study, FZ (125 mg/kg) was administered orally to Wistar rats for 3 days. Results of the Ames test using the S-9 fraction of rats treated with FZ showed a significant increase in the number of revertant colonies. Western blot analysis of hepatic CYP isozymes induced by FZ, revealed a remarkable induction of CYP1A1 apoprotein, but CYP1A2 and CYP2E1 apoproteins were not altered. In addition, the expression of CYP1A1 mRNA level in rats treated with FZ by RT-PCR was significantly enhanced by FZ treatment. We concluded that FZ is apparently mutagenic and induces transcription of the CYP1A1 isozyme, which metabolically activates numerous promutagens, in hepatocytes.

【 授权许可】

Unknown