Cytochrome P450 1A-Dependent Activities in Deer, Cattle and Horses

Journal of Veterinary Medical Science

Yoshinori IKENAKA¹  Kentaro Q. SAKAMOTO¹  Wageh Sobhy DARWISH¹  Shoichi FUJITA¹  Elsaid Abozeid ELDALY²  Marumi OHNO¹  Mayumi ISHIZUKA¹ 

[1] Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Food Control Department, Faculty of Veterinary Medicine, Zagazig University

关键词: CYP1A1;    cytochrome P450;    equine;    ethoxyresorufin O-deethylase;    ungulates;   

DOI  :  10.1292/jvms.09-0318

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(4)The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle and horses in comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody and identified that these activities were due to the CYP1A subfamily. Interspecies differences in the CYP1A-dependent activities were highly observed in this study. In particular, we found that the horse had the highest EROD and MROD activities among the examined animal species. In the kinetic analysis, the horses showed the highest Vmax and catalytic efficiency (Vmax/Km), followed by the cattle, deer and rats.

【 授权许可】

Unknown