Alterations in Interleukin-1β and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of p. Multocida Type b: 2 and its Lipopolysaccharide | Science Publications

American Journal of Animal and Veterinary Sciences

Lawan Adamu¹  Hazilawati Binti Hamzah¹  Faez Firdaus Jesse Abdullah¹  Yusuf Abba¹  Mohd Zamri B. Saad¹  Abdul Wahid B. Haron¹  Yusuf Ilyasu¹  M. L. Mohd-Azmi¹  Omar Suwaidan Ali¹ 

关键词: Lipopolysaccharides;    Pasteurella Multocida Type B2;    Cytokines;    Oral Route;    Graded Doses;    Mice;   

DOI  :  10.3844/ajavsp.2015.1.8

学科分类：兽医学

来源: Science Publications

【 摘 要 】

The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its LPS are associated with Haemorrhagic Septicaemia (HS) in mice as animal model. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n = 10) and these were inoculated with 0.4 mL of PBS pH 7.4 orally. The treatment groups (B; n = 25 and C; n = 25) were inoculated with P. multocida type B: 2 and lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B10¹, B10³, B10⁵, B10⁷ and B10⁹ for Pasteurella multocida and C10¹, C10³, C10⁵, C10⁷ and C10⁹ for LPS respectively. The mice were observed for 120 h post-inoculation. The concentration of IL-1β was significantly higher (p<0.001) in the B10⁷ of P. multocida type B: 2 and C10⁹ of LPS compared to the control group and the other treatment groups. The concentration of IL-6 was significantly higher (p<0.001) in the B10³ cfu of P. multocida type B: 2 and C10⁹ cfu of LPS compared to the control group and the other treatment groups. Interleukin-1β and interleukin-6 evaluated in this study were predominantly associated with HS in varying doses of 10¹, 10³, 10⁵, 10⁷ and 10⁹ in the whole cell and its LPS respectively. Therefore, the target of this study was to investigate on pro-inflammatory response in mice infected with graded doses of Pasteurella multocida serotype B: 2 and its LPS via the oral route of inoculation.

【 授权许可】

Unknown