Elevated Serum Bisphenol A Levels under Hyperandrogenic Conditions may be Caused by Decreased UDP-glucuronosyltransferase Activity

Endocrine Journal

Osamu TSUTSUMI²  Yumiko IKEZUKI²  Toshihiro FUJIWARA²  Yoshimasa KAMEI²  Tetsu YANO²  Yuji TAKETANI²  Yasushi TAKAI¹  Yutaka OSUGA²  Toru TAKEUCHI²  Mikio MOMOEDA² 

[1] Department of Obstetrics and Gynecology, Saitama Medical Center;Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

关键词: Bisphenol A;    UDP-glucuronosyltransferase;    UGT2B1;    Endocrine disruptor;    Androgen;   

DOI  :  10.1507/endocrj.K06-032

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(33)Cited-By(27)This study was performed to investigate the effect of androgen on the metabolism of bisphenol A (BPA), an endocrine disruptor, in order to clarify the mechanism of the higher levels of serum BPA in men and hyperandrogenemic women compared with normal women. Castrated female rats (OVX) were subcutaneously injected with testosterone propionate (TP) (0.01, 0.1, and 1 mg) every day for 2 weeks. Serum BPA concentrations in OVX rats showed a TP dose-dependent increase and were significantly higher at 0.1 and 1.0 mg of TP. The enzyme reaction of BPA glucuronidation in the rat liver microsomes showed that the ratio of glucuronide in the OVX rats was significantly reduced in a TP dose-dependent manner. Analysis of the mRNA expression of UDP-glucuronosyltransferase 2B1 (UGT2B1) by real-time quantitative RT-PCR revealed that the relative expression level of UGT2B1 mRNA showed a TP dose-dependent decrease. The results of enzyme analyses demonstrated that the ratio of BPA glucuronidation and the expression level of UGT2B1 mRNA were significantly lower under the hyperandrogenemic conditions. The clearance of BPA may be slowed in a TP dose-dependent manner, resulting in an increase of serum BPA concentration under hyperandrogenemic conditions.

【 授权许可】

Unknown