Immunohistochemical Study on the Delayed Progression of Epithelial Apoptosis in Follicle-Associated Epithelium of Rat Peyer's Patch

Journal of Veterinary Medical Science

Hiroshi KITAGAW¹  Tetsurou INAMOTO²  Hidenori MIYATA²  Sachiko ONISHI²  Kenkichi YAMAMOTO²  Wang-Mei QI²  Nobuhiko HOSHI¹  Katsuhiko WARITA³  Toshifumi YOKOYAMA¹ 

[1] Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University;Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University;Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

关键词: differentiation;    epithelial apoptosis;    M cell;    Peyer's patch;    rat;   

DOI  :  10.1292/jvms.69.1123

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(9)It is well known that some caspases in apoptosis is involved in determinant of terminal differentiation and maturation of various cells. Our previous study ultrastructurally clarified the differentiation into M cells from immature microvillous epithelial cells and the redifferentiation from M cells to microvillous epithelial cells in the follicle-associated epithelium (FAE) of rat Peyer's patch. In this study, the difference of epithelial apoptosis between the FAE of Peyer's patch and intestinal villi was immunohistochemically investigated in rat jejunoileum. As a result, cleaved caspase-3 was limited to several epithelial cells at the tip of FAE, whereas almost all of the epithelial cells were cleaved caspase-3 positive in intestinal villi. Cleaved caspase-9 was detected only in a few exfoliating or exfoliated epithelial cells of both FAE and intestinal villi. Nuclear DNA-fragmentation was detected only in several epithelial cells of the tip of FAE, while it was expressed from the middle regions in the intestinal villi. The DNase I expression of the epithelial cytoplasm was much weaker in FAE than in intestinal villi. Bcl-x expression was restricted in the apical cytoplasms of epithelial cells in the FAE, whereas it was restricted in whole cytoplasms in villous epithelial cells. These findings suggest that the progression of the apoptotic process in the epithelial cells of FAE is later than in the intestinal villi, so that the possibility of epithelial differentiation might be remained in the FAE, unlike in the intestinal villi.

【 授权许可】

Unknown