Special Sugar Expression on Apoptotic Epithelial Cells of Peyer's Patches and Intestinal Villi in Rat Small Intestine

Journal of Veterinary Medical Science

Tetsurou INAMOTO¹  Keigi CHIN¹  Sachiko ONISHI¹  Kenkichi YAMAMOTO¹  Midori YUJI¹  Wang-Mei QI¹  Nobuhiko HOSHI¹  Hiroshi KITAGAWA¹  Katsuhiko WARITA¹  Toshifumi YOKOYAMA² 

[1] Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University;Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

关键词: apoptosis;    bacterial colonization;    lectin histochemistry;    M cell;    Peyer's patch;   

DOI  :  10.1292/jvms.69.193

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(36)Cited-By(9)Our previous study clarified that the apical regions of both the follicle-associated epithelium (FAE) of Peyer's patches and the intestinal villi are the only adhesion sites of indigenous bacteria in rat jejuno-ileum. To survey the ligands against bacterial lectins, sugar expression patterns on epithelial cells were lectin-histochemically investigated using 21 lectins in the jejuno-ileal Peyer's patches of rats. As a result, (D-glcNAc)2-4, detected by Solanum tuberosum (STL) and by Lycopersicon esculentum (LEL), and β-D-gal(1-3)-D-galNAc detected by Peanut agglutinin (PNA), were strongly expressed on the brush borders of the apical regions of the FAE and the intestinal villi. On the other hand, neither sugar was expressed on the brush borders of the basal regions of both FAE and intestinal villi. The positive intensities for the lectins correlated with the progression of epithelial apoptosis in the FAE and in the intestinal villi. Moreover, the double staining with lectin histochemical method and the in situ nick end-labeling method could simultaneously detect the strong expression of both sugars and nuclear DNA fragmentation in epithelial cells at the late apoptotic stage. Other sugar expression patterns in the intestinal villi were similar with those in the FAE. There were no lectins specific for M cells in the FAE. From these findings, the possible sugars of ligands against some indigenous bacterial lectins, expressing specially on the apoptotic epithelial cells, might be narrowed down in rat jejuno-ileum.

【 授权许可】

Unknown