Identification and DNA Sequence Analysis of the Marek's Disease Virus Serotype 2 Genes Homologous to the Herpes Simplex Virus Type 1 UL20 and UL21

Journal of Veterinary Medical Science

Chieko KAI¹  Takeshi MIKAMI¹  Yoshihiro IZUMIYA¹  Eiji TAKAHASHI¹  Shinichi HATAMA¹  Takayuki MIYAZAWA¹  Hyung-Kwan JANG¹  Jin-Shun CAI¹  Kentaro KATO¹  Yoshinori TSUSHIMA¹ 

[1] Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

关键词: Marek's disease virus serotype 2;    nucleotide sequence;    ORF 273;    UL20;    UL21;   

DOI  :  10.1292/jvms.61.587

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(31)We determined 3, 135 bp of the nucleotide sequence located in an 8.5 kb EcoRI-E fragment in the unique long (UL) genome region of Marek's disease virus serotype 2 (MDV2), and identified UL20 and UL21 homologous genes of herpes simplex virus type 1 (HSV-1). The UL20 and UL21 homologous genes of MDV2 are arranged colinearly with the prototype sequence of HSV-1. In addition, an open reading frame (MDV2 ORF 273), which has been identified within the UL21 homologous gene of MDV2, has no apparent relation to any other known herpesvirus genes. Northern blot analysis and reverse transcriptase polymerase chain reaction confirmed the existance of RNA transcripts related to the UL20 and ORF 273 genes in MDV2-infected cells, except no transcript related to the UL21 gene being detected. The putative protein product of the MDV2 UL20 gene had a relatively low homology but that of the MDV2 UL21 gene had a moderate homology among herpesviruses. Further, the possible functions and features of the predicted proteins encoded within the sequenced region are discussed.

【 授权许可】

Unknown