Expression of genes SBP and leginsulin in contrasting soybean seed coats

Ciência Rural

Bahry, Carlos André¹  Zimmer, Paulo Dejalma² 

[1] Universidade Tecnológica Federal do Paraná, Dois Vizinhos, Brazil;Universidade Federal de Pelotas, Pelotas, Brazil

关键词: gene expression;    soybean coats;    qPCR;   

DOI  :  10.1590/0103-8478cr20140172

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:Evaluation of differential candidate gene expression in contrasting soybean seeds is an auxiliary tool in the partial elucidation of processes involved in seeds formation, as well as it contributes to the generation of new information that can be used in future research or in the development of r genetic superior constitutions. The aim of this study was to evaluate the expression of two candidate genes, SBP and leginsulin genes, possibly involved in seed quality, in contrasting coats of four soybean genotypes. Two cultivars of yellow soybeans were used, BMX Potência RR and CD 202, and two lines of black soybean, TP and IAC. Gene expression was evaluated using qPCR in seven stages of development from seed coats for four genotypes, at 25, 30, 35, 40, 45, 50, and 55 days after anthesis. The design was completely randomized, with three replications. Data were subjected to analysis of variance and means compared by Tu

【 授权许可】

Unknown