Reduction of Androgen Receptor mRNA Concentration by Testosterone in Mouse Submandibular Gland

Endocrine Journal

MASAZUMI NAGAI²  MINORU OTA²  TAKAYUKI NEMOTO²  TOMOYUKI MASUDA¹ 

[1] Department of Pathology, Iwate Medical University School of Medicine;Department of Biochemistry, Iwate Medical University School of Dentistry

关键词: Androgen;    Androgen receptor;    mRNA expression;    Submandibular gland;   

DOI  :  10.1507/endocrj.42.31

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(33)Cited-By(3)In this study, we examine the effect of testosterone on the steady-state concentration of androgen receptor (AR) mRNA of mouse submandibular gland. Northern blot analysis showed the expression of an AR-specific 10-kb transcript in the submandibular glands of both sexes. Quantitative analysis was done by means of reverse transcription-polymerase chain reaction (RT-PCR) with a non-radioactive label of digoxigenin. The RT-PCR demonstrated that the steady-state of AR mRNA concentration was less in males than in females. Moreover, AR mRNA increased in the castrated male mice, while the administration of testosterone reduced the AR mRNA in female mice. In conclusion, the amount of AR mRNA in mouse submandibular gland is reduced by androgen.

【 授权许可】

Unknown