Testosterone inhibits osteoclast formation stimulated by parathyroid hormone through androgen receptor

FEBS Letters

Chihara, Kazuo¹  Kaji, Hiroshi¹  Sugimoto, Toshitsugu¹  Chen, Qingxiang¹ 

[1] Third Division, Department of Medicine, Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650, Japan

关键词: Androgen;    Testosterone;    Osteoclast;    Osteoclast formation;    Parathyroid hormone;    Androgen receptor;   

DOI  :  10.1016/S0014-5793(01)02160-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Androgens play an important role in the regulation of bone metabolism in animals and humans. The present study was performed to investigate whether androgens would affect osteoclast formation stimulated by parathyroid hormone (PTH) in mouse bone cell cultures and its mechanism. Testosterone as well as α-dihydrotestosterone (DHT) concentration-dependently inhibited osteoclast formation induced by PTH-(1-34). 10⁻⁸ M ICI 182780, an estrogen receptor inhibitor, did not affect PTH-induced osteoclast formation antagonized by 10⁻⁸ M testosterone, although it completely antagonized the effects of 10⁻⁸ M 17β-estradiol. Moreover, 3 μM 4-androsten-4-ol-3,17-dione, an aromatase inhibitor, did not affect PTH-induced osteoclast formation antagonized by testosterone. Hydroxyflutamide, an androgen receptor antagonist, concentration-dependently antagonized the inhibitory effects of testosterone as well as DHT on PTH-stimulated osteoclast formation. In conclusion, the present study first demonstrated that testosterone inhibited osteoclast formation stimulated by PTH through the androgen receptor, but not through the production of intrinsic estrogen in mouse bone cell cultures.

【 授权许可】

Unknown