Immunization with Recombinant Surface Antigens p26 with Freund's Adjuvants against Babesia rodhaini Infection

Journal of Veterinary Medical Science

Ikuo IGARASHI²  Takeshi MIKAMI²  Kozo FUJISAKI²  Yoichiro IWAKURA³  Naoyoshi SUZUKI²  Atsushi SAITO¹  Urara ASABA²  Xuenan XUAN²  Hideyuki NAGASAWA²  Yoshitaka OMATA¹ 

[1] Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine;The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture;Institute of Medical Sciences, The University of Tokyo

关键词: Babesia rodhaini;    IFN-γ;    mouse;    recombinant antigen;    survival;   

DOI  :  10.1292/jvms.62.717

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(9)The surface proteins of Babesia rodhaini have previously been shown to induce a high degree of protective immunity. In the present study, one of those proteins, B. rodhaini antigen p26 was expressed in Escherichia coli and in insect cells infected with a recombinant baculovirus. These proteins were recognized by immune serum from a drug-cured BALB/c mouse. While BALB/c mice immunized with both recombinant antigens and Freund's adjuvants showed 40-100urvival rate against challenge infection with B. rodhaini, saponin failed to induce protection, although significant levels of B. rodhaini-specific antibodies were produced in both immunized mice (1:1,000-2,000 by indirect immunofluorescent antibody test). The immunization of IFN-γ-deficient mice with the recombinant proteins was not protective against B. rodhaini infection, indicating that IFN-γ is one of the important factors for the survival against lethal B. rodhaini infection.

【 授权许可】

Unknown