PROTECTION CONFERRED TO SWINE OFFSPRINGS INDUCED BY A VACCINE CANDIDATE OF PROTEIN SUBUNIT (E2) OF CLASSICAL SWINE FEVER VIRUS

Revista de Salud Animal

Vega, E²  Abeledo, María A²  Barrera, Maritza²  Bulnes, C²  Chong, Daine²  del Pilar Rodríguez, M¹  Teresa Frías, M²  Castell, Sara²  Figueredo, JM² 

[1] Centro de Ingeniería Genética y Biotecnología (CIGB), Habana, Cuba;Centro Nacional de Sanidad agropecuaria (CENSA), Mayabeque, Cuba

关键词: Classical Swine Fever;    maternal antibodies;    Margarita strain;    vaccine candidate of protein subunit (E2);   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

Four groups of pigs of 34 days old were conformed in order to evaluate the protective capacity conferred for maternal antibodies to offspring from mothers vaccinated with a vaccine candidate of protein subunit E2, against Classical Swine Fever virus (CSFV), obtained for adenoviral vector transduced to the mammary gland of goats, against challenge with a highly pathogenic strain (Margarita) at doses of 104 PID50 (infective doses 50% pigs). Groups 1 and 2 were conformed by five pigs with passively transferred maternal antibodies from mothers vaccinated with a vaccine candidate of protein subunit E2, and groups 3 and 4 (control groups ), conformed by three seronegative pigs to Classical Swine Fever (CSF), determined by neutralizing peroxidase linked assay (NPLA). The groups 1 and 3 were challenged by intranasal route and groups 2 and 4 by intramuscular route. The pigs with passively transferred maternal antibodies from mothers vaccinated and challenge by intranasal and intramuscular route were negative to viraemia and mortality; while those from the control groups were found sick and not protected for viraemia after challenge. The control group challenge for intramuscular route presented a smaller time of onset of the infectious process with respect to the group challenged by intranasal route.

【 授权许可】

CC BY-NC