Influenza H1N1pdm‐specific maternal antibodies offer limited protection against wild‐type virus replication and influence influenza vaccination in ferrets

Influenza and Other Respiratory Viruses

Amorsolo L. Suguitan Jr¹  James R. Zengel¹  Scott Jacobson¹  Stephanie Gee¹  Janet Cetz¹  Paulyn Cha¹  Zhongying Chen¹  Rosemary Broome¹ 

[1] MedImmune, Mountain View, CA, USA

关键词: Ferrets;    immunogenicity;    influenza;    live attenuated influenza vaccine;    maternal antibodies;    passive immunity;   

DOI  :  10.1111/irv.12220

来源: Wiley

【 摘 要 】

Abstract

Objective

The objective was to study passively acquired influenza H1N1 pandemic (H1N1pdm) maternal antibody kinetics and its impact on subsequent influenza infection and vaccination in ferrets during an outbreak of the H1N1pdm.

Design and main outcome measures

Infectivity of the H1N1pdm in the respiratory tract of ferrets was compared with the previous seasonal A/South Dakota/6/2007 (SD07, H1N1). Influenza-specific antibodies were quantitated and antibody-mediated protection against the homologous and heterologous H1N1 virus challenge infection was determined.

Results

H1N1pdm virus was approximately 10 times more infectious than SD07 in ferrets, replicated to higher viral titers in the upper respiratory tract and shed for a longer duration. Influenza-specific antibodies after natural infection persisted much longer in the circulation than passively acquired maternal antibodies. The protection conferred by the maternal antibodies was limited to the homologous virus strain and was ineffective against SD07 and H3N2 virus. Serum antibodies from maternal transmission or passive transfer interfered with homologous vaccine strain-mediated antibody responses in the ferret. A booster immunization was required to elicit a high level of antibody.

Conclusions

The findings support the rationale for a prime and boost immunization strategy in young children in whom maternal antibodies are present.

【 授权许可】

CC BY   
© 2013 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.