Screening of Vibrio isolates to develop anexperimental infection model in the Pacific oyster Crassostrea gigas

Diseases of Aquatic Organisms

Frédérique Le Roux¹  Franck C. J. Berthe¹  Mélanie Gay¹ 

关键词: Vibrio splendidus;    Virulence;    Agonism;    Antagonism;   

DOI  :  10.3354/dao059049

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: In an attempt to develop a reproducible experimental model of bacterial infection in Crassostrea gigas, oysters taken from very localised sub-populations suffering natural mortality outbreaks were used in cohabitation trials underlaboratory conditions. From these trials, a collection of Vibrio strains was isolated from moribund and healthy oysters. In a second step, strains were experimentally tested for virulence by means of injection into healthy oysters. This screeningrevealed a span of virulence among isolated strains from none to medium. When pooling injected strains, results suggest increased virulence. Vibrio strains may have additive/synergistic action leading to higher C. gigas mortality rates inexperimental challenges. Although the study initially aimed to develop a simple experimental model, a complex of interactions emerged between several bacterial strains during the pathogenic process in their molluscan host. Selected strains provide asuitable model of experimental disease for further studies and better understanding of bacterial interaction and pathogenesis in C. gigas.

【 授权许可】

Unknown