Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells

Endocrine Journal

YUKIHIRO SAKAMOTO¹  YASUMI SHINTANI¹  KAZUYO HARADA¹  TETSUYA GOTO¹  ZHONC-HUI LIU¹  MASAHIRO ABE¹  YUICHI FUJINAKA¹  SHIRO SAITO¹  CHEN-YU ZHANG¹ 

[1] First Department of Internal Medicine, School of Medicine, The University of Tokushima

关键词: Follistatin;    Activin;    LHRH;    FSH;    Anterior pituitary cells;    Immunoradiometric assay (IRMA);   

DOI  :  10.1507/endocrj.43.321

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(26)Cited-By(6)We demonstrated the release of follistatin, an activin-binding protein, from cultured rat anterior pituitary cells by measuring immunoreactive (ir-) follistatin in a specific immunoradiometric assay. Ir-follistatin release gradually increased in cultures over 1-18 days and reached its maximal level at 12-15 days of incubation. The basal ir-follistatin levels in the culture media increased about 3- (P< 0.01) and 5-fold (P<0.001) in 2 and 10% fetal calf serum for 6 days, respectively. LHRH and activin A caused an approximately 2.0- (P<0.05) and 1.8-fold (P<0.05) rise in ir-follistatin release, respectively, in contrast to the lack of significant FSH effects. The culture medium condensed on sulfate-cellulofine gel was resolved by polyacrylamide gel electrophoresis and blotted with anti-follistatin polyclonal antibody, resulting in at least three protein bands ranging from 35 to 50kDa under non-reducing conditions. These results indicated that follistatin is produced in anterior pituitary cells and that its secretion is regulated at least in part by LHRH and activin, implying an autocrine/paracrine role of activin and follistatin in the pituitary.

【 授权许可】

Unknown