Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Reproductive Biology and Endocrinology

Peter CK Leung¹  Chun Peng²  Victor TS Chen¹ 

[1] Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada;Department of Biology, York University, Toronto, Ontario, Canada

关键词: Competitive PCR;    Immortalized Extravillous Trophoblast Cells;    Activin Receptor;    Follistatin;    Activin;   

Others  :  1156974
DOI  :  10.1186/1477-7827-1-29

 received in 2003-03-07, accepted in 2003-03-24,  发布年份 2003

【 摘 要 】

Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

【 授权许可】

   
2003 Chen et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

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