Expression of Nitric Oxide Synthase Isoforms in the Human Placenta Is Not Altered by Labor

Endocrine Journal

Hiroaki ITOH¹  Norimasa SAGAWA¹  Kazuyo KAKUI¹  Mercy A. NUAMAH¹  Maki TAKEMURA¹  Shigeo YURA¹  Daizo KORITA¹  Shingo FUJII¹ 

[1] Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine

关键词: Guanosine 3';    5'-cyclic monophosphate (cGMP);    Nitric oxide synthase;    Parturition;    Pregnancy;    Trophoblast cells;   

DOI  :  10.1507/endocrj.50.535

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(54)Cited-By(8)Nitric oxide has various biological activities including smooth muscle relaxation, anti-inflammatory activity, anti-coagulatory activity. As the human placenta is known to express nitric oxide synthases, this study investigated the possible effect of labor on the expression of endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) in human placental tissues at term. Both eNOS and iNOS mRNA expression in placental tissues in labor were significantly higher than those in the amnion, chorion laeve, decidua vera and myometrium. The eNOS mRNA and protein expressions in placental tissues in labor (n = 12) were 1.6023 ± 0.1652 (eNOS/GAPDH, mean ± SEM) and 12.8 ± 1.3 arbitrary units (AU), respectively, which were similar to those not in labor (n = 10), 1.5806 ± 0.2042 (eNOS/GAPDH) and 11.4 ± 1.8 AU. The iNOS mRNA and protein expressions in the placental tissues in labor were 1.2831 ± 0.2436 (iNOS/GAPDH) and 10.7 ± 2.1 AU respectively, similar to those not in labor, 1.9254 ± 0.8004 (iNOS/GAPDH) and 13.3 ± 1.8 AU. The guanosine 3',5'-cyclic monophosphate (cGMP) cocentration in the placental tissues in labor was 23.6 ± 1.4 fmol/g wet tissue, similar to that not in labor, 26.1 ± 2.0 fmol/g wet tissue. These findings suggest that nitric oxide production in the human placenta is maintained during labor.

【 授权许可】

Unknown