T-588, a Cognitive Enhancer, Protects Against Sodium Nitroprusside-Induced Toxicity in Cultured Astrocytes

Journal of Pharmacological Sciences

Akemichi Baba¹  Tetsuo Fukushima¹  Kazuhiro Tanaka¹  Rie Hatanaka²  Patamawan Phuagphong¹  Toshio Matsuda² 

[1] Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University;Laboratory of Medicinal Pharmacology, Graduate School of Pharmaceutical Sciences, Osaka University

关键词: T-588;    nitric oxide;    mitochondria;   

DOI  :  10.1254/jphs.95.135

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(13)Cited-By(7)The effects of (1R)-1-benzo[b]thiophen-5-yl-2-[2-(diethylamino)ethoxy]ethan-1-ol hydrochloride (T-588), a cognitive enhancer, on sodium nitroprusside (SNP)-induced cytotoxicity were examined in cultured rat astrocytes. Treatment with 100 μM SNP for 72 h decreased cell viability and mitochondrial function assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenil tetrazolium bromide (MTT) reduction activity, mitochondrial transmembrane potential, and intracellular ATP level. T-588 at 100 μM prevented SNP-induced mitochondrial dysfunction and cell injury. Furthermore, T-588 increased MTT reduction activity without affecting cell proliferation in astrocytes. These results suggest that T-588 has a protective effect against SNP-mediated toxicity via improvement of mitochondrial dysfunction in astrocytes.

【 授权许可】

Unknown