Journal of Veterinary Medical Science | |
Evaluation of Different Cryoprotectants (CPAs) in Boar Semen Cryopreservation | |
Suhee KIM1  Dong-Beom JI1  Young-Jun LEE1  Yong-Jun KIM1  | |
[1] Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University | |
关键词: boar sperm; cryopreservation; DMA; DMSO; glycerol; | |
DOI : 10.1292/jvms.10-0345 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(15)Cited-By(5)This study was performed to evaluate the use of dimethylacetamide (DMA) and dimethyl sulfoxide (DMSO) in boar sperm cryopreservation. Semen from eight boars was cryopreserved following treatment with 3, 5, and 7% DMA and DMSO, and 3% glycerol (control). After thawing, sperm conventional parameters and membrane integrities were evaluated. There were no significant differences among different DMA concentrations in all evaluations. Membrane intactness were higher in 5% and 7% DMSO than 3% DMSO (P<0.05). Sperm motility of 5% DMSO was lower than that of 3% glycerol (P<0.005), and membrane intactness were lower in 5% DMA and DMSO than 3% glycerol (P<0.05). DMA and DMSO didn't improve sperm quality and glycerol remains the most useful for boar sperm cryopreservation.
【 授权许可】
Unknown
【 预 览 】
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RO201911300142922ZK.pdf | 482KB | download |