Cysteine peroxidase activity in rat blood plasma

Egyptian Journal of Biology

AV Razygraev¹ 

关键词: aminothiol;    stoichiometry;    5;    5';    -dithiobis(2-nitrobenzoic acid);    Ellman';    s reagent;   

DOI  :  

学科分类：生物科学（综合）

来源: Egyptian-English Society for Biological Sciences

【 摘 要 】

Early studies recognized that the human extracellular glutathione peroxidase may function cysteine peroxidase (cysteine:H 2 O 2oxidoreductase). The aim of the present study was the hypothesis of cysteine:H 2 O 2oxidoreductase activity in rat plasma. The rat plasma found to be able to accelerate greatly the H2O2-dependent oxidation of cysteine. The activity was a characteristic of a protein fraction precipitated at 30�?44% ammonium sulfate saturation, and the specific activity in protein fraction was significantly higher than in plasma. Cysteine:H 2 O 2oxidoreductase reaction stoichiometry (2:1 mole ratio) was detected for the catalyzed interaction between cysteine and H2O2. Estimation of cysteine peroxidase activity may be useful in research on the functioning of the antioxidant system. Keywords:aminothiol, stoichiometry; 5,5'-dithiobis(2-nitrobenzoic acid); Ellman's reagent

【 授权许可】

Unknown