Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan

Journal of Veterinary Medical Science

Masaji MASE¹  Katsushi KANEHIRA¹ 

[1] National Institute of Animal Health, 3–1–5 Kannondai, Tsukuba, Ibaraki 305–0856, Japan

关键词: Newcastle disease virus;    PCR;    REA;   

DOI  :  10.1292/jvms.12-0178

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(3)To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.

【 授权许可】

Unknown