期刊论文详细信息
Journal of Veterinary Medical Science | |
Simple Differentiation of Avirulent and Virulent Strains of Avian Paramyxovirus Serotype-1 (Newcastle Disease Virus) by PCR and Restriction Endonuclease Analysis in Japan | |
Masaji MASE1  Katsushi KANEHIRA1  | |
[1] National Institute of Animal Health, 3–1–5 Kannondai, Tsukuba, Ibaraki 305–0856, Japan | |
关键词: Newcastle disease virus; PCR; REA; | |
DOI : 10.1292/jvms.12-0178 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(15)Cited-By(3)To differentiate the avirulent from virulent strains of avian paramyxovirus serotype-1 (APMV-1, Newcastle disease virus), PCR and restriction endonuclease analysis (REA) was employed. Primer sequences were used to amplify a 766-base pair fragment that included the fusion protein cleavage site. REA of PCR products generated by Hin1 I and Apa I enabled the differentiation of avirulent field and vaccine strains from more virulent field strains of APMV-1 in Japan.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201911300107374ZK.pdf | 452KB | download |