Immunohistochemistry and molecular epidemiology of avian paramyxovirus 1 fromformalin-fixed and paraffin-embedded sections of Japanese doves (Columbalivia) affected with neurological signs

Journal of Veterinary Medical Science

Mitsutaka IKEZAWA¹  Yu YAMAMOTO¹  Kikuyasu NAKAMURA¹  Kunitoshi IMAI³  Hideo FUJIMORI²  Akiko KOYAMA²  Trinh Quang DAI³ 

[1] National Institute of Animal Health, 3�?1�?5 Kannondai, Tsukuba, Ibaraki 305�?0856, Japan;Tokyo Livestock Hygiene Center, 3�?19�?4 Fujimi-cho, Tachikawa, Tokyo 190�?0013, Japan;Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080�?8555, Japan

关键词: dove;    formalin-fixed paraffin-embedded section;    immunohistochemistry;    Newcastle disease virus;    PCR;   

DOI  :  10.1292/jvms.15-0004

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Four doves (Nos. 1�?4 birds) affected with neurological signs (ataxia, circling andtorticollis) were investigated pathologically and microbiologically. Viral isolation wastried from the tracheal and cloacal swabs of all 4 birds and from liver, spleen, kidney,heart, lung and brain of Nos. 1 and 2 birds. No viruses were isolated from 4 birds, butthey had high serum antibody titers against avian paramyxovirus 1 (APMV-1).Histologically, they had the characteristic histological changes of pigeon APMV-1infection; nonpurulent encephalitis and interstitial nephritis. Immununohistochemically,APMV-1 antigens were detected in the necrotic renal tubular epithelial cells of 1 bird ofthem (No. 3 bird). Detection of APMV-1 ribonucleic acid (RNA) from formalin-fixed andparaffin-embedded (FFPE) sections was attempted by reverse transcription-polymerase chainreaction (RT-PCR). Sequencing the RT-PCR product showed the virus RNA belonged to the sameAPMV-1 genotype (VI) as the strains isolated from the world previous cases of pigeonAPMV-1 infection. The RT-PCR of FFPE sections and sequencing of RT-PCR products are usefulfor molecular epidemiology of the virus when viral isolation from fresh samples isunsuccessful.

【 授权许可】

Unknown