Aquatic Biology | |
Isolation of cells from Atlantic sturgeon Acipenser oxyrinchus oxyrinchus and optimization of culture conditions | |
C. Kruse1  B. Grunow1  S. Noglick1  M. Gebert1  | |
关键词: Primary culture; Culture condition; Serum; Sturgeon; Teleost fish; | |
DOI : 10.3354/ab00383 | |
学科分类:生物科学(综合) | |
来源: Inter-Research | |
【 摘 要 】
ABSTRACT: We describe a method for fast and easy isolation of cells via trypsin digestion from larvae of Atlantic sturgeon Acipenser oxyrinchus oxyrinchus resulting in a stable, well-proliferating cell culture. The culture conditions for these cells were optimized with the aim of supporting the production of high amounts of biomass. To enhance cell growth and cell density, 4 different cultivation temperatures as well as commercially available carp serum (CS) and fetal calf serum (FCS) at different concentrations were tested and evaluated. Cell growth was measured via an impedance-based online cell-monitoring system (xCELLigence). These results showed the best cultivation temperature to be at 25°C and a media composition of Dulbecco’s modified Eagle’s medium (DMEM) supplemented with either 10 or 20% FCS or 5% CS. The cells were stable in the process of long-term cultivation over 33 passages and could be cryo-preserved. Immunocytochemical analysis revealed that the cells expressed proteins of different blastodermic layers. Ectodermic glia fibrilliary acid protein, vigilin (mRNA transport protein), and pan cytokeratin were abundant. This fast-growing cell culture provides an important tool for research on Atlantic sturgeon populations.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201911300080889ZK.pdf | 943KB | download |