Culture Conditions for Mouse Pancreatic Stem Cells

Cell Medicine

Toshiyoshi Fujiwara¹  Hirofumi Noguchi²  Masami Watanabe³  Yasufumi Noguchi⁴  Issei Saitoh⁵  Hitomi Usui Kataoka⁶ 

[1] #Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;*Department of Surgery, Clinical Research Center, Chiba-East Hospital, National Hospital Organization, Chiba, Japan;§Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan;¶Department of Socio-environmental Design, Hiroshima International University, Hiroshima, Japan;†Department of Pediatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan;‡Department of Primary Care and Medical Education, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

关键词: Mouse pancreatic stem cells;    Culture condition;    Embryonic stem cells;    Embryonic stem (ES) medium;    Feeder cells;    Pancreatic islet transplantation;   

DOI  :  10.3727/215517913X666495

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Recently, mouse pancreatic stem cells have been isolated from adult mouse pancreata. However, these pancreatic stem cells could be maintained only under specific culture conditions with lot-limited fetal bovine serum (FBS). For the efficient isolation and maintenance of mouse pancreatic stem cells, it is important to identify culture conditions that can be used independent of the FBS lot. In this study, we evaluated the culture conditions required to maintain mouse pancreatic stem cells. The mouse pancreatic stem cells derived from the pancreas of a newborn mouse, HN#101, were cultured under the following conditions: 1) Dulbecco's modified Eagle's medium (DMEM) with 20% lot-limited FBS, in which mouse pancreatic stem cells could be cultured without changes in morphology and growth activity; 2) complete embryonic stem (ES) cell media; and 3) complete ES cell media on feeder layers of mitomycin C-treated STO cells, which were the same culture conditions used for mouse ES cells. Under culture conditions #1 and #3, the HN#101 cells continued to form a flat “cobblestone” monolayer and continued to divide actively beyond the population doubling level (PDL) 100 without growth inhibition, but this did not occur under culture condition #2. The gene expression profile and differentiated capacity of the HN#101 cells cultured for 2 months under culture condition #3 were similar to those of HN#101 cells at PDL 50. These data suggest that complete ES cell media on feeder layers could be useful for maintaining the undifferentiated state of pancreatic stem cells.

【 授权许可】

CC BY