Increased Induction of Inducible Nitric Oxide Synthase Expression in Aortae of Type 2 Diabetes Rats

Journal of Pharmacological Sciences

Tomoyuki Nakano¹  Yuji Takahashi¹  Ichiro Wakabayashi¹ 

[1] Department of Environmental and Preventive Medicine, Hyogo College of Medicine, Japan

关键词: artery;    glucose;    lipopolysaccharide;    nitric oxide synthase;    type 2 diabetes;   

DOI  :  10.1254/jphs.FP0080010

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(28)Cited-By(9)The aim of this study was to determine whether the pathway of inducible NO synthase (iNOS) in blood vessels is changed by type 2 diabetes. Lipopolysaccharide (LPS)-induced nitric oxide (NO) production and expression of iNOS and effects of LPS on phenylephrine-induced contractile force were compared in aortae isolated from Goto-Kakizaki (G-K) diabetes rats and aortae isolated from control Wistar rats. Both LPS-stimulated nitrite generation and iNOS expression levels were significantly higher in aortae from G-K rats than in those from control rats. Phenylephrine-induced contractile force in the presence of LPS was significantly lower in aortae from G-K rats than in those from control rats, while contractile force in the absence of LPS was comparable in the diabetic and control groups. On the other hand, incubation of aortae in high glucose-containing medium did not affect the LPS-stimulated nitrite accumulation and iNOS expression and the phenylephrine-induced contractile force, regardless of the presence of LPS. These results suggest that LPS-induced NO production through the iNOS pathway is increased and subsequent attenuation of contractile force by excess NO is enhanced in arteries of rats with type 2 diabetes.

【 授权许可】

Unknown