Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus

FEBS Letters

Hernández-Perera, Octavio²  Rodrigo, José¹  Cueto, Mercedes³  Lamas, Santiago²  Golvano, Maria Pilar³  Bentura, Maria Luisa¹  Martín, Raquel³ 

[1] Departamento de Neuroanatomía Comparada, Instituto de Neurobiología Santiago Ramón y Cajal, CSIC, 28002 Madrid, Spain;Departamento de Estructura y Función de Proteínas, Centro de Investigaciones Biológicas, CSIC, 28006 Madrid, Spain;Departamento de Fisiología y Bioquímica Vegetal, Centro de Ciencias Medioambientales, CSIC, Serrano 115 dpdo, 28006 Madrid, Spain

关键词: Nitric oxide synthase;    Lupinus albus;    Rhizobium;    Root;    Root nodule;    l-NMMA;    NG-monomethyl-l-arginine;    NOS;    nitric oxide synthase;    LPS;    lipopolysaccharide;   

DOI  :  10.1016/S0014-5793(96)01232-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NO is a widespread messenger molecule in physiology. We were interested in investigating whether an NO-generating system could be present in plants. NO and l-[¹⁴C]citrulline were synthesized by roots and nodules of Lupinus albus in an l-arginine-dependent manner. l-[¹⁴C]Citrulline production was inhibited by N^G-monomethyl-l-arginine, a nitric oxide synthase antagonist, in a competitive way. NADPH-diaphorase activity was localized in the vascular bundles in root and nodules, and also in the nodule infected zone. This staining was significantly reduced in the presence of N^G-monomethyl-l-arginine. These results indicate the presence of a putative nitric oxide synthase in plants.

【 授权许可】

Unknown