Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India

Diseases of Aquatic Organisms

Iddya Karunasagar¹  Indrani Karunasagar¹  S. K. Otta¹  B. Joseph¹  Anirban Chakraborty¹  G. Shubha¹ 

关键词: White spot syndrome;    Virus;    Polymerase chain reaction;    Cultured shrimp;    Wild crustaceans;   

DOI  :  10.3354/dao038067

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A study was carried out to evaluate polymerase chain reaction (PCR) for the detection of white spot syndrome virus (WSSV) in postlarvae and broodstock of Penaeus monodon and in potential crustacean carriers from India. Primer pairsdesigned for WSSV affecting Penaeus japonicus (WSSV PJ) and P. monodon (WSSV PM) were used. Both the primer pairs gave positive assay results for crustaceans showing gross signs of WSSV infection. However, all grossly healthypostlarvae and broodstock gave negative results with both the WSSV PJ and WSSV PM primer pairs by non-nested PCR. Some of the grossly normal specimens were positive for WSSV by nested PCR using WSSV PM primers. So also was water from shrimp culture pondsexperiencing WSSV outbreaks. In addition, 1 sample of Artemia nauplii was positive for WSSV PM by nested PCR. Among crab samples examined, a few samples of Scylla serrata and Sesarma oceanica from around the pond areaand marine crabs such as Matuta planipes and Charybdis lucifera from trawl catches were positive for WSSV, indicating that wild marine crabs could be reservoirs of WSSV infection. The results indicated that WSSV carriers arecommon in natural shrimp stocks and other crustaceans in India.

【 授权许可】

Unknown