Up to Species-level Community Analysis of Human Gut Microbiota by 16S rRNA Amplicon Pyrosequencing

Bioscience of Microbiota, Food and Health

Kenji SONOMOTO³  Takashi KURAKAWA²  Kangting CHEN¹  Huang NINXIN¹  Kazunori MATSUDA²  Hirokazu TSUJI²  Jiahui JIANG³  Jiro NAKAYAMA³  Yuan-Kun LEE¹  Koichi WATANABE² 

[1] Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;Yakult Central Institute for Microbiological Research, Japan;Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Japan

关键词: pyrosequence;    16S rRNA gene;    human gut microbiota;   

DOI  :  10.12938/bmfh.32.69

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

References(13)Cited-By(7)Pyrosequencing-based 16S rRNA profiling has become a common powerful tool to obtain the community structure of gastrointestinal tract microbiota, but it is still hard to process the massive amount of sequence data into microbial composition data, especially at the species level. Here we propose a new approach in combining the quantitative insights into microbial ecology (QIIME), Mothur and ribosomal database project (RDP) programs to efficiently process 454 pyrosequence data to bacterial composition data up to the species level. It was demonstrated to precisely convert batch sequence data of 16S rRNA V6-V8 amplicons obtained from adult Singaporean fecal samples to taxonomically annotated biota data.

【 授权许可】

Unknown