Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue

Cell Medicine

Nana Ebi^1¹  Mariko Masamoto^1²  Yoshihito Tamaki^1³  Erika Makishi^1⁴  Eri Hamada^1⁵  Chika Miyagi-Shiohira^1⁶ 

[1] Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan^1;Department of Socio-environmental Design, Hiroshima International University, Hiroshima, Japan^5;Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan^4;Division of Morphological Pathology, Department of Basic Laboratory Sciences, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan^6;Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan^3;Okayama Saidaiji Hospital, Okayama, Japan^2

关键词: islet transplantation;    islet isolation;    islet purification;    bottle purification;    continuous density gradient;   

DOI  :  10.1177/2155179017733090

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification.

【 授权许可】

CC BY