Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification

Cell Medicine

Chika Miyagi-Shiohira^1¹  Yoshiki Nakashima^1²  Eri Hamada^1³  Yoshihito Tamaki^1⁴  Nana Ebi^1⁵ 

[1] Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan^1;Department of Surgery, Okayama Saidaiji Hospital, Okayama, Japan^2;Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan^4;Division of Morphological Pathology, Department of Basic Laboratory Sciences, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan^5;Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan^3

关键词: bottle purification;    islet transplantation;    islet isolation;    islet purification;    tissue loading;   

DOI  :  10.1177/2155179018781343

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification.

【 授权许可】

CC BY