A Comparison of Pancreatic Islet Purification using Iodixanol with University of Wisconsin Solution and with Na-Lactobionate and Histidine Solution

Cell Medicine

Yoshiki Nakashima^1¹  Eri Hamada^1²  Yoshihito Tamaki^1³  Nana Ebi^1⁴  Chika Miyagi-Shiohira^1⁵ 

[1] Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa Prefecture, Japan^1;Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama Prefecture, Japan^4;Division of Morphological Pathology, Department of Basic Laboratory Sciences, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa Prefecture, Japan^5;Division of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan^3;Okayama Saidaiji Hospital, Okayama Prefecture, Japan^2

关键词: Islet transplantation;    islet isolation;    islet purification;    bottle purification;    continuous density gradient;   

DOI  :  10.1177/2155179018775071

学科分类：生物科学（综合）

来源: Cognizant Communication Corporation

【 摘 要 】

Purification of pancreatic islets is an important step in islet isolation for islet transplantation. In this study, to investigate how a solution composed mainly of Na-lactobionate and histidine (HL) influences the purification of islets, iodixanol was added to a purified solution for porcine islet isolation. A solution (IU) made by adding iodixanol to University of Wisconsin solution and a solution (IHL) made by adding iodixanol to HL solution were used to evaluate the islet isolation performance. We noted no significant differences between the two purification methods with regard to the islet yield, survival rate or purity, score, or stimulation index. These results show that IHL solution is as useful as IU solution for islet purification.

【 授权许可】

CC BY