Hypocholesterolaemic effect of whole-grain highland hull-less barley in rats fed a high-fat diet

The British Journal of Nutrition

Guannan Li^2¹  Jiaxin Song^1²  Xuejuan Xia^1³  Jiong Zheng^1⁴ 

[1] College of Biotechnology,Southwest University,Chongqing 400715,People’s Republic of China^2;College of Food Science,Southwest University,Chongqing 400715,People’s Republic of China,^3;College of Food Science,Southwest University,Chongqing 400715,People’s Republic of China^1;Laboratory of Quality And Safety Risk Assessment for Agro-products on Storage and Preservation (Chongqing),Ministry of Agriculture,400715,People’s Republic of China^4

关键词: Hull-less barley;    Cholesterol metabolism;    Cellular biomarkers;    Gene expression;    Dietary fibres;   

DOI  :  10.1017/S0007114518000831

学科分类：卫生学

来源: Cambridge University Press

【 摘 要 】

Whole-grain highland hull-less barley (WHLB) contains high amounts of bioactive compounds that potentially exhibit cholesterol-lowering effects. This study investigated the hypocholesterolaemic effect of WHLB. A total of seventy-two male Sprague–Dawley rats were divided into four groups and were fed with the normal control diet, high-fat diet (HFD) and HFD containing low or high dose (10 or 48·95 %) of WHLB. High dose of WHLB significantly decreased the organ indexes of liver and abdominal fat and lipid levels of plasma and liver in HFD rats. The lipid regulation effect of WHLB, which was reconfirmed through hepatocyte morphologic observation, was accompanied by a large excretion of bile acids in the small intestinal contents and the faeces. Real-time PCR analyses, which were further reconfirmed through Western blot analyses, revealed that a high dose of WHLB significantly enhanced the hepatic expressions of AMP-activated protein kinase α, cholesterol 7α-hydroxylase, LDL receptor, liver X receptor, and PPARα and decreased the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase. It also enhanced the ileal expression of farnesoid X receptor and resulted in the decrease of expression of apical sodium-dependent bile acid transporter. WHLB exhibited hypocholesterolaemic effects mainly by inhibiting cholesterol synthesis, cholesterol accumulation in peripheral tissue, and bile acid reabsorption and by stimulating bile acid synthesis.

【 授权许可】

CC BY