The effect of inhibition of cholesterol esterification on the fate of cholesterol derived from HDL in rat hepatocyte monolayers

FEBS Letters

Jackson, Brian¹  Suckling, Keith E.¹  Botham, Kathleen M.²  Sampson, William J.¹ 

[1] Department of Cellular Pharmacology, Smith Kline & French Research Ltd, The Frythe, Welwyn AL6 9AR England;Department of Veterinary Basic Sciences, Royal Veterinary College, Royal College Street, London NW1 0TU, England

关键词: Bile acid;    HDL;    Acyl-CoA:cholesterol acyltransferase;    Lipoprotein;    Cholesterol metabolism;    (Rat hepatocyte);   

DOI  :  10.1016/0014-5793(88)80893-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat HDL₂ is known to stimulate bile acid synthesis in rat hepatocyte monolayers. The intracellular fate of the cholesterol derived from the HDL₂ was studied using the inhibitor of cholesterol esterification, Sandoz compound 58-035. Rat HDL₂ added to rat hepatocyte monolayers caused a stimulation of cholesterol esterification of 32%. This stimulation could be inhibited by 58-035. A small significant increase in bile acid synthesis was also observed in cells in the presence of HDL₂, confirming our earlier observations. 58-035 prevented this increase. These observations imply that cholesterol entering the cell from HDL₂ is first esterified and can only enter the substrate pool for bile acid synthesis after subsequent intracellular hydrolysis.

【 授权许可】

Unknown