Bile acid binding in plasma: the importance of lipoproteins

FEBS Letters

Lugli, R.¹  Salvioli, G.¹  Gigliotti, G.¹  Pradelli, J.M.¹ 

[1] University of Modena, Ospedale Estense, Viale Vittorio Veneto, 9, 41100 Modena, Italy

关键词: Bile acid;    Hydrophobicity;    Protein;    Lipoprotein binding;    VLDL;    very low density lipoproteins (d < 1.006 g/ml);    LDL;    low density lipoproteins (d = 1.030–1.063 g/ml);    HDL;    high density lipoproteins (d = 1.063–1.210 g/ml);    d > 1.210 g/ml;    lipoprotein-free fraction;    BS;    bile salts;    BA;    bile acids;    DCA;    deoxycholic acid (3α-;    12α-dihydroxy-5β-cholanoic acid);    CDCA;    chenodeoxycholic acid (3α;    7α-dihydroxy-5β- cholanoic acid);    HDCA;    hyodeoxycholic acid (3α;    6α-dihydroxy-5β-cholanoic acid);    UDCA;    ursodeoxycholic acid (3α;    7β-dihydroxy-5β-cholanoic acid);    CA;    cholic acid (3α-;    7α;    12α -trihydroxy-5β-cholanoic acid);    UCA;    ursocholic acid (3α;    7β;    12α-trihydroxy-5β-cholanoic acid);    GLC;    gas liquid chromatography;    TLC;    thin layer chromatography;    HPLC;    high performance liquid chromatography;   

DOI  :  10.1016/0014-5793(85)81257-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bile acid (BA) hydrophobicity, evaluated by the octanol-water partition coefficient, decreases along the series deoxycholic acid - chenodeoxycholic acid - hyodeoxycholic acid - ursodeoxycholic acid - cholic acid (CA) - ursocholic acid (UCA). In vitro experiments carried out using dialysis techniques (to determine the maximum BA binding) and ultrafiltration of plasma pre-incubated with 0.1 mM BA (to assess the distribution of BA between the different lipoprotein fractions) showed that the maximum binding of BA to plasma and lipoproteins follows the same order of hydrophobicity. The fraction not bound to proteins, greater with the hydrophilic BA (UCA and CA), is distributed in the lipoprotein fractions and in particular in high density lipoproteins.

【 授权许可】

Unknown