Effect of the bile acid taurolithocholate on cell calcium in saponin‐treated rat hepatocytes

FEBS Letters

Combettes, Laurent²  Dumont, Micheline¹  Claret, Michel²  Erlinger, Serge¹  Berthon, Brigitte² 

[1] Unité de Recherches INSERM U. 24, Hôpital Beaujon Centre Abrami, 92118 Clichy, France;Unité de Recherche INSERM U. 274, Université Paris-Sud Bât. 443, 91405 Orsay Cedex France

关键词: Ca2+;    Bile acid;    Endoplasmic reticulum;    Neomycin;    (Rat liver);    quin2;    2-[[2-bis(carboxymethyl)amino-5-methylphenoxyl] methyl]-6-methoxy-8-bis(carboxymethyl)-amino-quinoline;    quin2/AM;    quin2 tetraacetoxymethyl ester;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;   

DOI  :  10.1016/0014-5793(88)80889-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neomycin was used to assess the involvement of Ins (1,4,5)P₃ in the Ca²⁺ release from the endoplasmic reticulum induced by the bile acid taurolithocholate. In saponin-permeabilized rat hepatocytes, neomycin via its ability to bind Ins (1,4,5)P₃ abolished the release of Ca²⁺ induced by added Ins (1,4,5)P₃. In contrast, it did not alter the Ca²⁺ release initiated by the bile acid. In intact cells, neomycin had no effect on the [Ca²⁺]_i rises promoted by taurolithocholate and vasopressin. It is suggested that the effect of taurolithocholate in liver is not mediated by Ins (1,4,5)P₃ but results from a primary action on endoplasmic reticulum.

【 授权许可】

Unknown