Neomycin: a specific drug to study the inositol‐phospholipid signalling system?

FEBS Letters

Deeney, Jude T.¹  Prentki, Marc¹  Joseph, Suresh K.¹  Matschinsky, Franz M.¹ 

[1] Department of Biochemistry and Biophysics and Diabetes Research Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA

关键词: Neomycin Aminoglycoside antibiotic Ca2+ release Inositol phospholipid Inositol phosphate;   

DOI  :  10.1016/0014-5793(86)80343-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neomycin, an antibiotic previously thought to interact specifically with inositol-containing phospholipids, was found to inhibit IP₃-mediated Ca²⁺ release from the intracellular stores of permeabilized insulinoma and liver cells. This inhibition could be relieved by increasing the IP₃ concentration. Radiolabelled IP₃ was found to bind tightly to columns prepared from neomycin covalently attached to glass beads. ATP was also bound by these colums. It is concluded that neomycin acts in biological systems as a weak anion exchanger and is therefore unsuitable for use as a specific tool to study the role of inositol phospholipids in intracellular signalling.

【 授权许可】

Unknown