The Efficacy of Photosensitizers on Mycelium Growth, Mycotoxin and Enzyme Activity of Alternaria spp.

Jordan Journal of Biological Sciences

Kholoud M. Alananbeh¹ 

关键词: Alternaria spp.;    Photosensitizer;    Cellulase;    Pectinase;    Mycotoxin.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Vegetables are subjected to heavy yield losses in quality and quantity as a result of various diseases caused by dematiaceous fungi. However, the use of fungicides is hazardous to humans, animals, and the environment. In this study, emphasis is given to other methods of disease control through the employment of photodynamic treatments such as photosensitizers which are considered safer, more economical, and eco-friendly. The objective of the current study was to evaluate the possibility of using photodynamic inactivation against Alternaria alternata, the causal agent of tomato blight. Samples of tomato, squash, pepper and cucumber were collected from different fields, and the isolated fungi were identified morphologically and molecularly. The inhibitory doses of the photosensitizer regarding the growth of A. alternata were studied. The results showed that 100 μg/mL of toluidine blue-O (TBO) was effective inhibiting Alternaria spp. with a significance variation among different species of Alternaria. The A. alternata isolated from tomato was more resistant to TBO and less sensitive to light compared to other isolates. The effect of the photosensitizer on cellulase and pectinase as well as the production of alternariol mycotoxin were studied. The results showed that the photosensitizer was inhibitory for enzyme activity and the alternariol production especially in the presence of light. In conclusion, photosensitizers can be used for treating plant pathogenic fungi such as Alternaria spp.

【 授权许可】

CC BY