EVALUATION OF PHENOLIC CONTENTS (QUANTITATIVE AND QUALITATIVE) AND ANTIOXIDANT ACTIVITIES IN DIFFERENT PHYSIOLOGICAL PHASES OF Genista saharae COSS. & DUR. GROWING IN THE SAHARA OF ALGERIA

Universitatea din Oradea. Analele. Fascicula Biologie

Atef CHOUIKH¹ 

关键词: Genista saharae;    Polyphenols;    Flavonoids;    Antioxidant activity;    test DPPH;    Hemolysis test;    HPLC.;   

DOI  :  

学科分类：生物科学（综合）

来源: Editura Universitatii din Oradea / University of Oradea Publishing House

【 摘 要 】

To evaluate the natural products in the desert plants, antioxidant activity and phenolic contents of Genista saharae (Fabaceae) growing in Oued Souf region (Algerian desert) were determined during different phases of its development. The results of the yield of methanolic extracts showed that the highest value was recorded in the extracts of the floral phase (8.6%) and the lowest was in the extracts of the fruiting phase (5.16 %). As we observed the relationship in the quantitative content of polyphenols and flavonoids, were recorded with the highest value of polyphenols at the first vegetative phase estimated as 1.33±0.018mg EAG/g Ex and 0.772 ±0.088mg EQu/g Ex, and the lowest value was in fruiting phase 0.443±0.078mg EAG/g Ex and 0.21±0.036mg EQu/g Ex. While the results of antioxidant activity in the free radical revealed that, the extract of the second vegetative phase exceeded with a best inertial capacity than the other extracts (IC50= 0.247 mg/ml). As for concerning the hemolysis test, the finding indicated at distinction of extract the first vegetative phase with a lower hemolysis 31.26%. Using the HPLC, the qualitative analysis of extracts showed quantitative and qualitative differences concerning the presence of some phenolic compound such as: Gallic acid, Caffeic acid, ρ-Coumaric acid, Vanillin and Rutin in four extracts. According to results obtained it seemed that there is a disparity in the phenolic content in terms of the different stages of growth. This seemed obvious in antioxidant activity results that can be the change in the quality and concentration of the phenolic compounds between different stages of growth.

【 授权许可】

Unknown