HPLC method for simultaneous determination of metronidazole and preservatives in vaginal gel formulation

Acta Chromatographicae

Maslarska Vania¹  Tsvetkova Boyka² 

[1] Medical University of Sofia, Faculty of Pharmacy, Department of Chemistry, 1000 Sofia;Medical University of Sofia, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 1000 Sofia

关键词: Liquid chromatography;    metronidazole;    methylparaben;    propylparaben;    validation;    vaginal gel;   

DOI  :  10.1556/1326.2017.00098

学科分类：化学（综合）

来源: Akademiai Kiado Rt.

【 摘 要 】

A high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection for simultaneous determination of metronidazole, methylparaben, and propylparaben in vaginal gel formulation was described. The chromatography was carried out on a C18 (250 mm × 4.6 mm, 5 μm) column with acetonitrile and 0.3% phosphoric acid solution (20:80 v/v) modified by 0.1% triethylamine as mobile phase, at a flow rate of 1.0 mL min−1, with detection at 260 nm. Under these chromatographic conditions, the obtained retention times were approximately 3.43 min for metronidazole, 5.17 min for propylparaben, and 15.12 min for methylparaben.

【 授权许可】

Unknown