Oxysterols Profile in Zebrafish Embryos Exposed to Triclocarban and Propylparaben—A Preliminary Study

International Journal of Environmental Research and Public Health

Stefano Lorenzetti¹  Giulia Caioni²  Annamaria Iannetta³  Dario Compagnone³  Monia Perugini³  Michele Amorena³  Anton Vremere³  Manuel Sergi³  Federico Fanti³  Carmine Merola³ 

[1] Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità—ISS, 00161 Rome, Italy;Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy;Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, 64100 Teramo, Italy;

关键词: oxysterols;    propylparaben;    triclocarban;    zebrafish embryos;    toxicity;   

DOI  :  10.3390/ijerph19031264

来源: DOAJ

【 摘 要 】

Oxysterols have long been considered as simple by-products of cholesterol metabolism, but they are now fully designed as bioactive lipids that exert their multiple effects through their binding to several receptors, representing endogenous mediators potentially involved in several metabolic diseases. There is also a growing concern that metabolic disorders may be linked with exposure to endocrine-disrupting chemicals (EDCs). To date, there are no studies aimed to link EDCs exposure to oxysterols perturbation—neither in vivo nor in vitro studies. The present research aimed to evaluate the differences in oxysterols levels following exposure to two metabolism disrupting chemicals (propylparaben (PP) and triclocarban (TCC)) in the zebrafish model using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Following exposure to PP and TCC, there were no significant changes in total and individual oxysterols compared with the control group; however, some interesting differences were noticed: 24-OH was detected only in treated zebrafish embryos, as well as the concentrations of 27-OH, which followed a different distribution, with an increase in TCC treated embryos and a reduction in zebrafish embryos exposed to PP at 24 h post-fertilization (hpf). The results of the present study prompt the hypothesis that EDCs can modulate the oxysterol profile in the zebrafish model and that these variations could be potentially involved in the toxicity mechanism of these emerging contaminants.

【 授权许可】

Unknown