Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells

The Korean Journal of Parasitology

Pu Reum Sun^1¹  Hei Gwon Choi^1²  Fei Fei Gao^1³ 

[1]Department of Medical Science & Infection Biology, Chungnam National University, School of Medicine, Daejeon 34134, Korea^1

[2]Department of Medical Science & Medical Education, Chungnam National University, School of Medicine, Daejeon 34134, Korea^3

[3]Institute of Immunology, Taishan Medical College, Tai’an 271-000, Shandong, China^2

关键词: T. gondii;    reactive oxygen species;    diphenyleneiodonium;    N-acetyl-L-cysteine;    ARPE-19 cell;   

DOI  :  10.3347/kjp.2019.57.2.83

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and H₂O₂ levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.

【 授权许可】

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