Early signaling components in ultraviolet‐B responses: distinct roles for different reactive oxygen species and nitric oxide

FEBS Letters

A.-H.-Mackerness, Soheila²  Jordan, Brian¹  John, C.Fred²  Thomas, Brian² 

[1] Division of Soil, Plant and Ecological Sciences, Lincoln University, P.O. Box 84, Canterbury, New Zealand;Department of Plant Genetics and Biotechnology, Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK

关键词: Gene expression;    Nitric oxide;    Reactive oxygen species;    Signal transduction;    Ultraviolet-B;    chs;    chalcone synthase;    CAT;    catalase;    DDC;    N;    N-diethyldithiocarbamate;    DPI;    diphenyleneiodonium;    JA;    jasmonic acid;    IM;    imidazole;    Lhcb;    light harvesting complex binding proteins;    L/D-NAME;    N G-monomethyl-1-arginine;    NO;    nitric oxide;    NOS;    NO synthase;    PAL;    phenylalanine ammonia lyase;    PDF1.2;    defencin;    PR-1;    pathogenesis-related-1;    PTIO;    2-phenyl-4;    4;    5;    5-tetramethylimidazolin-1-oxyl-3-oxide;    ROS;    reactive oxygen species;    SA;    salicylic acid;    SHAM;    salicylhydroxamic acid;    SOD;    superoxide dismutase;    UV-B;    ultraviolet-B;   

DOI  :  10.1016/S0014-5793(01)02103-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nature and origin of the reactive oxygen species (ROS) involved in the early part of Ultraviolet-B (UV-B)-induced signaling pathways were investigated in Arabidopsis thaliana using a range of enzyme inhibitors and free radical scavengers. The increase in PR-1 transcript and decrease in Lhcb transcript in response to UV-B exposure was shown to be mediated through pathways involving hydrogen peroxide (H₂O₂) derived from superoxide (O₂ ⁻). In contrast, the up-regulation of PDF1.2 transcript was mediated through a pathway involving O₂ ⁻ directly. The origins of the ROS were also shown to be distinct and to involve NADPH oxidase and peroxidase(s). The up-regulation of Chs by UV-B was not affected by ROS scavengers, but was reduced by inhibitors of nitric oxide synthase (NOS) or NO scavengers. Together these results suggest that UV-B exposure leads to the generation of ROS, from multiple sources, and NO, through increased NOS activity, giving rise to parallel signaling pathways mediating responses of specific genes to UV-B radiation.

【 授权许可】

Unknown