Induction of Angiogenesis by Malarial Infection through Hypoxia Dependent Manner

The Korean Journal of Parasitology

Hyunsu Kim^1^2¹  Eun-Ji Ko^1^2²  Mi-Kyung Park^1³  Jin-Ok Jo^1⁴  Kyung-Yoon Jeon^1⁵ 

[1] Anti-Aging Research Center and Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 47227, Korea^4;Department of Biological Science, Pusan National University, Busan 46241, Korea^2;Department of Parasitology and Genetics, Kosin University College of Medicine, Busan 49267, Korea^1;Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu 41944, Korea^5;Department of Parasitology, College of Medicine, Pusan National University, Busan 50612, Korea^3

关键词: Plasmodium berghei;    malaria;    hypoxia;    angiogenesis;    HIF1α;    VEGF;   

DOI  :  10.3347/kjp.2019.57.2.117

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Malarial infection induces tissue hypoxia in the host through destruction of red blood cells. Tissue hypoxia in malarial infection may increase the activity of HIF1α through an intracellular oxygen-sensing pathway. Activation of HIF1α may also induce vascular endothelial growth factor (VEGF) to trigger angiogenesis. To investigate whether malarial infection actually generates hypoxia-induced angiogenesis, we analyzed severity of hypoxia, the expression of hypoxia-related angiogenic factors, and numbers of blood vessels in various tissues infected with Plasmodium berghei . Infection in mice was performed by intraperitoneal injection of 2×10⁶ parasitized red blood cells. After infection, we studied parasitemia and survival. We analyzed hypoxia, numbers of blood vessels, and expression of hypoxia-related angiogenic factors including VEGF and HIF1α. We used Western blot, immunofluorescence, and immunohistochemistry to analyze various tissues from Plasmodium berghei -infected mice. In malaria-infected mice, parasitemia was increased over the duration of infection and directly associated with mortality rate. Expression of VEGF and HIF1α increased with the parasitemia in various tissues. Additionally, numbers of blood vessels significantly increased in each tissue type of the malaria-infected group compared to the uninfected control group. These results suggest that malarial infection in mice activates hypoxia-induced angiogenesis by stimulation of HIF1α and VEGF in various tissues.

【 授权许可】

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