Evolutionarily distant I domains can functionally replace the essential ligand-binding domain of Plasmodium TRAP

eLife

Julia Sattler¹  Friedrich Frischknecht²  Sarah Goellner³  Dennis Klug⁴  Mirko Singer⁴  Jessica Kehrer⁴  Léanne Strauss⁴  Timothy A Springer⁴  Chafen Lu⁴ 

[1] Department of Molecular Virology, Heidelberg University Medical School, Heidelberg, Germany;Experimental Parasitology, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, München, Germany;Université de Strasbourg, CNRS UPR9022, INSERM U963, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France;Integrative Parasitology, Center for Infectious Diseases, Heidelberg University Medical School, Heidelberg, Germany;

关键词: Plasmodium berghei;    malaria;    motility;    invasion;    integrin;    adhesion;   

DOI  :  10.7554/eLife.57572

来源: DOAJ

【 摘 要 】

Inserted (I) domains function as ligand-binding domains in adhesins that support cell adhesion and migration in many eukaryotic phyla. These adhesins include integrin αβ heterodimers in metazoans and single subunit transmembrane proteins in apicomplexans such as TRAP in Plasmodium and MIC2 in Toxoplasma. Here we show that the I domain of TRAP is essential for sporozoite gliding motility, mosquito salivary gland invasion and mouse infection. Its replacement with the I domain from Toxoplasma MIC2 fully restores tissue invasion and parasite transmission, while replacement with the aX I domain from human integrins still partially restores liver infection. Mutations around the ligand binding site allowed salivary gland invasion but led to inefficient transmission to the rodent host. These results suggest that apicomplexan parasites appropriated polyspecific I domains in part for their ability to engage with multiple ligands and to provide traction for emigration into diverse organs in distant phyla.

【 授权许可】

Unknown