Caspases and matrix metalloproteases facilitate collective behavior of non-neural ectoderm after hindbrain neuropore closure

BMC Developmental Biology

Naomi Shinotsuka¹  Yoshifumi Yamaguchi²  Kenichi Nakazato³ 

[1] Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan;Hibernation Metabolism, Physiology and Development Group, Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan;Theoretical Biology Laboratory, RIKEN, Wako, Japan

关键词: Apoptosis;    Caspases;    Matrix metalloproteases;    Neural tube closure;    Live-imaging;   

DOI  :  10.1186/s12861-018-0175-3

学科分类：生物科学（综合）

来源: BioMed Central

【 摘 要 】

Mammalian brain is formed through neural tube closure (NTC), wherein both ridges of opposing neural folds are fused in the midline and remodeled in the roof plate of the neural tube and overlying non-neural ectodermal layer. Apoptosis is widely observed from the beginning of NTC at the neural ridges and is crucial for the proper progression of NTC, but its role after the closure remains less clear. Here, we conducted live-imaging analysis of the mid-hindbrain neuropore (MHNP) closure and revealed unexpected collective behavior of cells surrounding the MHNP. The cells first gathered to the closing point and subsequently relocated as if they were released from the point. Inhibition of caspases or matrix metalloproteases with chemical inhibitors impaired the cell relocation. These lines of evidence suggest that apoptosis-mediated degradation of extracellular matrix might facilitate the final process of neuropore closure.

【 授权许可】

CC BY