Genetic diversity of cytochrome P450 2A with different metabolic activities in domestic cats

The Journal of Veterinary Medical Science

Tomoko AMANO¹  Souta SUGIYAMA²  Yasuhiro UNO³ 

[1] College of Agriculture, Food and Environment Sciences, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan;Shin Nippon Biomedical Laboratories, Ltd., Kainan, Wakayama 642-0017, Japan

关键词: cytochrome P450;    domestic cat;    polymorphism;    xenobiotic;   

DOI  :  10.1292/jvms.19-0107

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Knowledge of genetic polymorphisms of cytochrome P450 (CYP), the most important xenobiotic metabolizing enzyme, is very limited in cats. Preliminarily, we investigated genetic polymorphisms in CYP2A13, one of the major CYP isoforms in the liver and lung. Four synonymous and three non-synonymous polymorphic variants were identified in feline CYP2A13 in domestic cats in Japan, without an obvious major type. Metabolic parameters, Km and Vmax, of coumarin hydroxylation of CYP2A13 were shown to range within two times for the identified non-synonymous polymorphic variants by using heterologous coexpression system in Escherichia coli. The results confirmed the polymorphic nature of CYP2A13 as a basis for effective application of medicines and prevention of adverse reactions in treatment of domestic cats.

【 授权许可】

Unknown